Graves病CD4^+CD25^+ T调节细胞水平的变化

Changes in count of circulating CD4^+CD25^+ T regulator cells in patients with Graves disease

目的研究Graves病患者CD4+CD25+T调节细胞水平的变化。方法应用流式细胞仪检测59例初发甲亢患者和29名健康人外周血CD4+CD25+、CD4+CD25+highT淋巴细胞数量,其中17例患者进行了为期3个月治疗前后的观察。结果甲亢患者与正常人之间CD4+CD25+、CD4+CD25+highT淋巴细胞数无明显差异。与正常对照组比较,32例伴有甲状腺微粒体抗体阳性患者CD4+CD25+highT淋巴细胞数增加,差异有显著性。17例患者经丙基硫氧嘧啶治疗前后CD4+CD25+、CD4+CD25+highT淋巴细胞数并无明显差异。结论Graves病患者CD4+CD25+T调节细胞数量无明显变化,治疗前后亦无明显改变,但部分甲状腺相关免疫指标异常的Graves病患者CD4+CD25+highT淋巴细胞数增加,表明这部分患者有较严重细胞免疫紊乱。

Objective To investigate the change cells in patients with Graves disease. Methods in count of circulating CD4^＋ CD25^＋ regulatory T （Treg） The counting of CD4^＋ CD25^＋ and CD4^＋ CD25^＋ high regulatory T cells was examined by using a flow cytometer in 59 patients with initial Graves disease and 29 healthy volunteers. Results There was no significant difference in count of CD4^＋ CD25^＋ and CD4^＋ CD25^＋high regulatory T cells between the patient group and the control group. Thirty-two patients with positive microsomal antibody had significantly higher proportion of CD4^＋ CD25 ^＋high regulatory T cells compared with that of the control group. There was no significant difference in the proportion of CD4^＋CD25^＋ and CD4^＋CD25^＋high regulatory T cells before and after treatment with PTU in 17 patients. Conclusion There are no significant changes in the proportion of CD4^＋CD25^＋ regulatory T cells not only in patients with Graves diseases but also before and after treatment with PTU. However, compared with healthy control, higher count of CD4^＋ CD25^＋high regulatory T cells has been observed in some patients with initial Graves disease who have abnormal thyroid-related immune tests, indicating more severe cell immune disorder in these patients. More special T cell tests are needed in further research of autoimmune disease.