摘要
目的研究炎热清颗粒中栀子苷的含量测定方法,制定和完善其质量标准。方法采用Phenomenex ODS-3C18柱(250×4.60mm,5μm);以0.1%磷酸溶液-乙腈-异丙醇(90:15:0.5)为流动相;检测波长为238nm。结果对照品在4.0~48μg·mL^-1浓度范围内呈线性关系,r=0.9997,加样回收率平均为101.16%(n=5),RSD为0.52%。结论本方法简便、准确、重复性好,可用于炎热清颗粒中栀子苷的含量测定。
OBJECTIVE To establish the TLC and HPLC determination methods of jasminoidin in Yanreqing Granules. METHODS Jasminoidin were identified by HPLC;The condition of HPLC was Phenomenex ODS-3 C18 column(250 × 4.6mm,5μm) ,mobile phase of 0. 1% H3PO4 aqueous solution-- acetonitrile-- isopropanol(90 : 15 : 0. 5), with a flow rate of 1.0mL · min^-1 ,UV detector at 238nm. RESULTS The calibration curve was linear ( r =0. 9997) within the range of 4. 0- 48μg · mL^-1 for jasminoidin. The average recovery was 101.16% ( n =5), RSD was 0.52%. CONCLUSION The method was simple,accurate and reproducible,It may be used for the assay of jasminoidin in Yanreqing Granules.
出处
《齐鲁药事》
2009年第10期594-596,共3页
qilu pharmaceutical affairs