HPLC法测定炎热清颗粒中栀子苷的含量

Determination of jasminoidin in Yanreqing Granules by HPLC

目的研究炎热清颗粒中栀子苷的含量测定方法，制定和完善其质量标准。方法采用Phenomenex ODS-3C18柱（250×4．60mm，5μm）；以0．1％磷酸溶液-乙腈-异丙醇（90：15：0．5）为流动相；检测波长为238nm。结果对照品在4．0～48μg·mL^-1浓度范围内呈线性关系，r=0．9997，加样回收率平均为101．16％（n=5），RSD为0．52％。结论本方法简便、准确、重复性好，可用于炎热清颗粒中栀子苷的含量测定。

OBJECTIVE To establish the TLC and HPLC determination methods of jasminoidin in Yanreqing Granules. METHODS Jasminoidin were identified by HPLC;The condition of HPLC was Phenomenex ODS-3 C18 column（250 × 4.6mm,5μm） ,mobile phase of 0. 1% H3PO4 aqueous solution-- acetonitrile-- isopropanol（90 ： 15 ： 0. 5）, with a flow rate of 1.0mL · min^-1 ,UV detector at 238nm. RESULTS The calibration curve was linear （ r =0. 9997） within the range of 4. 0- 48μg · mL^-1 for jasminoidin. The average recovery was 101.16% （ n =5）, RSD was 0.52%. CONCLUSION The method was simple,accurate and reproducible,It may be used for the assay of jasminoidin in Yanreqing Granules.