复方愈疡散对糖尿病大鼠难愈合创面Smad3 TGF-β1的影响

Influence on Smad3 TGF-β_1 in Chronicity Rehabilitation of Surface of Wound by Therapeutic Methods of Fufangyuyang Pulvis

目的:研究补虚祛瘀法促进慢性难愈合创面愈合的机理。方法:采用改良付氏大鼠模型为研究对象,分为模型对照组(A组)、复方愈疡散组(B组)、补虚方组(C组)、祛瘀方组(D组),运用免疫组化法分别研究各组第10天时创面瘢痕组织中Smad3、TGF-β1的变化。结果:第10天时,B组Smad3、TGF-β1表达低于A组,C组Smad3的表达低于A组。提示复方愈疡散在实验性大鼠难愈合创面肉芽组织形成期,对TGF-β1→Smad3信号转导通路的调控起着重要作用。

Objective： To research the mechanism of chronicity raw surface coalesced by the method of reinforce dificiency and removing blood stasis.Methods：We divided rats into four groups：A（group of hpysiologic saline）,B（group of Fufangyuyang Pulvis）,C（group of reinforce difieiency）,D（group of removing blood stasis） ,and then treated them by different therapeutic methods.The changes of TGF- β1, Smad3 by immunohistochemistry at 10th day were observed.Results：The express of Smad3 and TGF-β1 of group B was lower than that of group A,while the express of Smad3 of group c was lower than that of group A.Conclusion： The method of reinforce deficiency and remodeling blood stasis can modulate TGF-β1→Smad3 signal transduetion.