重寄生菌粉红粘帚霉SS-1-1菌株的发酵条件优化

Optimization of hyperparasite (Gliocladium roseum strain SS-1-1) fermentation conditions

采用正交试验和单因素试验对粉红粘帚霉(Gliocladium roseum)SS-1-1菌株发酵条件的优化进行了研究。SS-1-1菌株最佳发酵培养基配方为:麸皮40g/L,玉米粉50g/L,豆饼粉20g/L,KH2PO4 0.5g/L;最佳发酵条件为:培养基初始pH值为4.5,发酵温度为28℃,摇床转速为200r/min,最佳培养时间为5d。

Optimaization of hyperparasite （Gliocladium rose um strain SS-1-1） fermentation conditions was studied via single factor and orthogonal test design. The optimum medium compostion for strain SS-1-1 was： bran 40 g/L, corn flour 50 g/L, bean cake powder 20 g/L, KH2PO4 0.5g/L. And the optimum fermentaion conditions for maximum biomass concentration were： 5 days cultivation under 28℃ with initial pH 4.5 and rotation speed 200 r/min