微波提取-高效液相色谱法测定土壤中痕量阿特拉津

Determination of atrazine in soil by using microwave assisted extraction coupled with high performance liquid chromatography

目的：采用微波提取,高效液相色谱-紫外检测法测定土壤中阿特拉津。方法：分别考察了萃取溶剂种类、用量、温度、时间等因素对萃取率的影响。结果：考察了洗脱条件、柱流速及柱温对阿特拉津分离的影响;方法最低检测限为1.00 ng/kg,相对标准偏差小于1.00%,回收率为71.3%-91.9%。结论：结果表明该方法能够满足土壤样品中阿特拉津的检测要求。

Objective：To develop an analytical method for the determination of atrazine in soil by using microwave assisted extraction（MAE） coupled with high performance liquid chromatography（HPLC）.Methods：To improve the extraction efficiency,experimental parameters of the extraction,including extraction solvent and its amount,extraction temperature and time were investigated.Results：The chromatographic conditions,such as the mobile phase,flow rate and column temperature,were optimized.The detection limits of the method was 1.00 ng/kg,and the relative standard deviation（RSD） was less than 1.00%.The recoveries of the method were from 71.3% to 91.9%.Conclusion：This method is fast,convenient,sensitive and suitable for the determination of atrazine in soil.