蛋白芯片联合检测多种肿瘤标志物在恶性肿瘤诊断和筛查中的应用

Protein biochip application in diagnosis of malignant tumor with combined measurement of multiple serum tumor markers

目的:探讨蛋白芯片联合检测多种肿瘤标志物在恶性肿瘤的诊断和筛查中的价值。方法:采用多种肿瘤标志物蛋白芯片系统测定分析308例恶性肿瘤患者,172例良性疾病患者和926例健康体检者血清中的12种肿瘤标志物(CA199、CEA、AFP、CA242、CA125、CA153、NSE、β-HCG、f-PSA、PSA、HCH、FER)的含量。结果:恶性肿瘤组的阳性率(73.05%)显著高于良性疾病组(31.98%)和健康体检组(11.56%)(P<0.001);除胰腺癌外,多种肿瘤标志物联合检测对肺癌、胃癌、结直肠癌、食管癌、肝癌和乳腺癌检出阳性率均显著高于其单一肿瘤标志物检测(P<0.01)。结论:蛋白芯片联合检测血清多种肿瘤标志物可以显著提高恶性肿瘤诊断的敏感性,对肿瘤的早期诊断具有一定的应用价值,尤其适用于体检筛查。

Objective：To investigate the diagnostic values of multi-tumor markers protein biochip detective system in malignant tumors.Methods：Using the multiple tumor marker protein biochip detective system to determine and analyze the serum levels of 12 common tumor markers,including CA199,CEA,AFP,CA242,CA125,CA153,NSE,β-HCG,f-PSA,PSA,HCH and FER,in 308 cancer patients,172 patients with benign disease and 926 healthy examinations.Results：The positive rates were 73.05%,31.98% and 11.56% in malignant tumors,being disease and healthy groups,respectively.The malignant tumors group had significantly higher positive rate than that of the controls（P0.001）.Except for pancreatic cancer,combined measurement had higher positive rate for lung cancer,gastric carcinoma,colonic cancer,liver cancer,esophageal cancer and breast cancer than single tumor marker（P0.01）.Conclusion：Combined measurement of multiple serum tumor markers using protein biochip technique can significantly increase the diagnostic sensitivity for malignant tumors,and can be used as a routine assay for detection the tumor markers especially for physical examination.