HBsAg阴性献血者血清HBV-DNA检测的意义

Significance of HBV-DNA detection in serum of HBsAg negative blood donors

目的：探讨HBV-DNA检测对于输血安全的重要性。方法：用ELISA法对2000例HBsAg阴性献血员进行HBV血清标志物检测,同时采用荧光定量PCR法检测HBV-DNA。结果：2000例HBsAg阴性献血员样本中有9例为HBV-DNA阳性,检出率为0.45%,对HBV-DNA的定量测定表明,其含量在103-106拷贝数/ml。结论：为尽可能减少输血后HBV感染的发生,HBV-DNA检测对于保证输血安全有重要意义。

Objective：To investigate the significance of HBV-DNA detection in HBsAg negative blood donors for blood transfusion safety.Methods：The markers and DNA of HBV were determined by ELISA and PCR in 2000 samples from HBsAg negative blood donors.Results：Totally 2000 samples from HBsAg negative blood donors were examined,the positive rate of HBV-DNA was 0.45%.The viral loads were 103-106 copies/ml.Conclusion：In order to reduce HBV infection after blood transfusion,it is very important for blood transfusion safety to detect HBV-DNA in HBsAg negative blood donors.