摘要
目的:探讨HBV-DNA检测对于输血安全的重要性。方法:用ELISA法对2000例HBsAg阴性献血员进行HBV血清标志物检测,同时采用荧光定量PCR法检测HBV-DNA。结果:2000例HBsAg阴性献血员样本中有9例为HBV-DNA阳性,检出率为0.45%,对HBV-DNA的定量测定表明,其含量在103-106拷贝数/ml。结论:为尽可能减少输血后HBV感染的发生,HBV-DNA检测对于保证输血安全有重要意义。
Objective:To investigate the significance of HBV-DNA detection in HBsAg negative blood donors for blood transfusion safety.Methods:The markers and DNA of HBV were determined by ELISA and PCR in 2000 samples from HBsAg negative blood donors.Results:Totally 2000 samples from HBsAg negative blood donors were examined,the positive rate of HBV-DNA was 0.45%.The viral loads were 103-106 copies/ml.Conclusion:In order to reduce HBV infection after blood transfusion,it is very important for blood transfusion safety to detect HBV-DNA in HBsAg negative blood donors.
出处
《中国卫生检验杂志》
CAS
2009年第11期2653-2654,共2页
Chinese Journal of Health Laboratory Technology