应用半对数回归ELISA法调查孕妇麻疹抗体水平

Investigation on measles antibody level among pregnant woman using semi-logarithm regression ELISA

目的:了解仁寿地区孕妇麻疹免疫水平,为制定麻疹预防和麻疹疫苗免疫策略提供依据。方法:在常规ELISA法的基础上,以阳性参考血清倍比稀释度的对数为横坐标,与其对应的OD/CUT OFF值进行线性回归,制定校准曲线。根据被测血清标本的OD/CUT OFF值推算其麻疹抗体滴度。结果:212名孕妇麻疹IgG阳性率为39.62%,抗体几何平均滴度(GMT)为1:121.87。结论:仁寿地区孕妇麻疹抗体水平低,应对育龄期妇女实施选择性麻疹疫苗免疫策略,并将其纳入免疫规划管理,以有效提高育龄妇女麻疹免疫水平,控制麻疹的发生。

Objective：To understand measles immunity level among Renshou pregnant womem to provide scientific basis for measles prevention and measles immunization strategy.Methods：The logarithm of serial doubling dilution of the positive control serum was made as abscissa,semi-logarithm regression was made with their corresponding OD/CUT OFF values,and the measles antibody titer of serum specimens according to their OD/CUT OFF values in the standard curve was calculated.Results：The positive rate of measles antibody IgG was 39.62%,geometric mean titer（GMT）was 1：121.87.Conclusion：Measles antibody level among Renshou pregnant womem was lower,measles immunization should be strengthened for the target population,it must be brought into the line of programmed immunization,measles immunity level can be improved effectively,the epidemic of measles can be controlled.