PTEN与Survivin在人皮肤血管瘤组织中的表达及意义

Significance and Expression of PTEN and Survivin in Human Dermal Hemangioma

目的探讨PTEN与Survivin在血管瘤发生、发展过程中的表达及意义。方法应用免疫组织化学方法和RT-PCR法检测了皮肤血管瘤增生期、退化期以及正常皮肤组织中PTEN和Survivin的表达水平。结果①免疫组织化学结果:PTEN在增生期血管瘤内皮细胞的表达低于退化期,差异有显著性意义(P<0.05);退化期血管瘤内皮细胞PTEN表达水平高于正常皮肤组织,差异有显著性意义(P<0.05)。Survivin在增生期血管瘤内皮细胞的表达明显高于退化期血管瘤内皮细胞和正常皮肤组织血管内皮细胞(均P<0.01),而Survivin在正常皮肤组织血管内皮细胞的表达与退化期血管瘤内皮细胞的表达差异无显著性意义(P>0.05)。②RT-PCR结果:在退化期血管瘤和正常皮肤组织中均有明显的PTEN mRNA表达,而增生期血管瘤中PTEN mRNA的表达较弱;在增生期血管瘤中有明显的Survivin mRNA表达,而退化期血管瘤和正常皮肤组织中Survivin mRNA均无表达。结论PTEN和Survivin参与了血管瘤的发生、发展和退化,PTEN通过诱导内皮细胞凋亡而促进血管瘤由增生向退化的转变,Survivin通过抑制内皮细胞凋亡而促进血管瘤的增生,两者在血管瘤的发生发展中发挥协同效应。

Objective To study the expression of PTEN and Survivin,and investigate the mechanism and significance of them in different phases of human hemangiomas.Methods The expression of PTEN and Survivin was detected by using immuno-histochemical technique,and that of PTEN mRNA and Survivin mRNA by using reverse transcription polymerase chain reaction（RT-PCR）in proliferating,involuting human hemangiomas and normal skin tissues.Results ①The expression of PTEN in the endothelial cells of involuting hemangiomas was significantly higher than in proliferating hemangiomas（P〈0.05）and normal skin tissues（P〈0.05）.The expression of PTEN in the endothelial cells of involuting hemangiomas was higher than in normal skin tissues（P〈0.05）.The expression of Survivin was significantly higher in the endothelial cells of proliferating hemangiomas than in involuting hemangiomas and normal skin tissues（P〈0.01）,but there was no significant difference in the expression of Survivin between the endothelial cells in involuting hemangiomas and normal skin tissues（P〉0.05）.②The expression of PTEN mRNA was strong in involting hemangiomas and normal skin tissues,but weak in proliferating hemangiomas.The expression of Survivin mRNA was significantly increased in proliferating hemangiomas,and no Survivin mRNA was detected in involuting hemangiomas and normal skin tissues.Conclusion It is suggested that both PTEN and Survivin may take part in the genesis,development,and involution.PTEN promoted the switching from proliferation to involution in hemangiomas through inducing the apoptosis of endothelial cells.Survivin accelerated the proliferation of hemangiomas by inhibiting the apoptosis of endothelial cells.