降低茶树组织培养中外植体褐化程度的研究

Effect of Different Culture Conditions on Browning Rate of Explants in Tissue Culture of Tea Trees

为降低茶树组培过程中外植体褐化程度,从外植体表面消毒时间、切割方式、转瓶间隔时间、培养基类型、激素配比、抗褐化剂和吸附剂的使用方面进行了综合试验。结果表明：表面消毒最适时间为7～8 min;接种外植体带腋芽叶片保留2/5,3 d转瓶1次降低褐化的效果较好;经此处理的外植体培养在1/2MS＋BA 2 mg/L＋NAA 0.1 mg/L＋Vc 1.0 g/L＋AC 1.0 g/L的培养基中,生长良好,褐化率由86%下降为41%。

The sterilized time and cutting pattern of explants,interval time for transferring another bottle,medium types,different hormone combination,anti-browning agents and absorption agents were studied to reduce the browning rate of explants in tissue culture of tea tree.The results showed that the browning rate could reduce from 86 % to 41 % when the explants with 2/5 leaf and a axillary bud were sterilized by 0.1 % mercury solution for 7-8 min,the explants were transferred into another bottle every 3d and the treated explants were cultured on 1/2MS ＋BA 2 mg/L＋NAA 0.1 mg/L＋Vc 1.0 g/L＋AC 1.0 g/L.