幼龄小鼠小肠组织内参基因的选择

The selection of reference genes in young mouse small intestine

选择B2 M、ACTB、GAPDH、SDHA、HPRT1和ARBP共6个内参基因,研究其在幼龄小鼠小肠组织内的表达情况.结果表明:6个内参基因均可获得特异性扩增产物;经geNorm程序和NormFinder分析,6个内参基因稳定度由高到低为SDHA=HRPT1>ARBP>ACTB>GAPDH>B2 M;基因表达的稳定值分别为0.006(SDHA)、0.006(HRPT1)、0.007(ARBP)、0.018(ACTB)、0.029(GAPDH)、0.111(B2 M),最终筛选出SDHA和HRPT1 2个内参基因适合用于校正目的基因的表达.

Reference genes are widely used for normalization of the expression level of mRNA in RT-PCR,but the expression of reference gene is influenced by physiological stage,tissues or cells,and the conditions of experiments.The purpose of the present study was to investigate the expression of B2M,ACTB,GAPDH,SDHA,HPRT1 and ARBP by RT-PCR in young mouse small intestine.By geNorm and normFinder analysis,SDHA and HPRT1 were finally determined as suitable reference genes and could be used to normalize mRNA levels between different samples.