拟南芥ATMYB2转录因子的cDNA序列分析及其细胞定位

cDNA sequence analysis and its cell localization of Arabidopsis thaliana ATMYB2 transcription factors

根据GenBank中收录的拟南芥At Myb2基因(GenBank登录号:AK229140)的cDNA序列设计1对引物,对拟南芥中叶片提取的总RNA进行扩增和克隆,并将拟南芥ATMYB2蛋白氨基酸序列进行同源性比较和蛋白定位分析.结果表明:At Myb2基因cDNA全长为816 bp,编码272个氨基酸和1个终止密码子,具有2个典型的MYB类转录因子基因的DNA结合区,分别为23-70、79-118位氨基酸,属于典型的R2,R3-MYB转录因子;经过氨基酸比对发现,拟南芥ATMYB2蛋白与水稻的ATMB18蛋白同源性最高,为44.60%.对拟南芥At Myb2基因进行RT-PCR扩增,结果表明基因片段未发生任何突变;通过拟南芥ATMYB2与EGFP形成融合蛋白对AT-MYB2进行了定位,发现ATMYB2蛋白在细胞核内能够表达,符合作为转录因子的表达特征.

Based on the cDNA sequence of AtMyb2 gene from GenBank,a pair of primers were designed and used to amplify AtMyb2 gene from total RNA abstracted from Arabidopsis thaliana leaves,meanwhile,the homology comparison and cell localization on the amino acid sequence of AtMyb2 protein were carried out.The results showed that cDNA of AtMyb2 gene was 816 bp,which encoding 272 amino acid and one stop codon,and had 2 typical DNA-binding domains for MYB genes.The homology comparison showed that the homology of ATMYB2 proteins between Arabidopsis thaliana and O.sativa was 44.60 %.The RT-PCR on AtMyb2 gene showed that there was no mutation in this gene.Cell localization of ATMYB2 protein showed that the ATMYB2 protein expressed in the cellular nucleus.