附子镇痛作用量效关系的实验研究

The Experimental Study on Dose-Effect Relationship of Chinese Medicine Prepared Aconite Root on Effect of Analgesia

目的:研究附子剂量与其镇痛作用的相关性,探索附子用于镇痛时的最佳用量。方法:急性毒性实验测定附子水煎醇沉液半数致死量(LD50),采用扭体法、热板法观察不同剂量附子的镇痛作用,绘制量效关系曲线。结果:水煎醇沉液LD50为31.24g/kg。附子对热板致痛的镇痛作用与模型组比较无明显差异;附子对冰醋酸致痛的小鼠有明显镇痛作用,且在0.75～6g/kg剂量范围内呈明显剂量依赖性,剂量与扭体抑制率之间线性方程为:Y=0.0696X+0.2222,r=0.8301。结论:附子仅在0.75～6g/kg剂量范围内与其镇痛作用存在正相关性。

Objective： To inquire the Dose- Effect relationship of Chinese Medicine prepared aconite root （PAT） on effect of reviving the Yang for resuscitation, and to provide the experimental basis for security administration. Methods： PAT were extracted with the water decoction - alcohol sedimentation method. We measured LDso. The analgesic effect was tested by the hot - plate test and writhing method. Results ： The result of acute toxicity testing was LD50 = 31.24g/kg. Analgesia experiments of hot plate method indicated, rather than black mice, the analgesic threshold of mice couldn＇t be elevated by taking PAT. There was direct correlation between dose of PAT and effect of analgesia in acetic acid inducing mice within 0.75 -6g/kg dose range ; The linear relationship between the prepared aconite root dosage and inhibition ratios of acetic acid： Y = 0.0696X ＋ 0.2222, r = 0. 8301 o Conclusion ： The effect of Analgesia was notable and showed dosage - effect relationship within 0.75 - 6g/kg dose range .