灯盏细辛注射液治疗大鼠慢性肾功能不全氮质血症的实验研究

Experimental Study of Dengzhanxixin Injection in the Treatment of Rats with Chronic Renal Insufficiency and Azotemia

目的:对灯盏细辛注射液治疗慢性肾功能不全的作用做动态观察,探求该药改善肾功能的药理机制。方法:健康成年SD大鼠进行5/6肾切除,除假手术组外,选用符合慢性肾功能不全氮质血症条件动物60只,随机分为模型组、尿毒清组、灯盏细辛大剂量组、灯盏细辛中剂量组及灯盏细辛小剂量组,每组12只。于4周、8周和12周动态观察血尿素氮和血肌酐水平,12周时采用ELISA法测定肾组织TNF-α含量,分光光度法进行SOD定量测定,采用SAS8.2e软件进行统计学处理。结果:灯盏细辛注射液各剂量组均有改善肾功能不全的作用,以大剂量组最明显,呈现出量-效关系,但作用弱于尿毒清(P<0.05);灯盏细辛治疗使TNF-α下降和SOD含量升高(P<0.05)。结论:灯盏细辛注射液可改善慢性肾功能不全,其机制可能是通过降低TNF-α水平、促进自由基清除剂SOD的合成。

Objective： Dengzhanxixin injection in treating chronic renal failure role of dynamic observation were done to explore the pharmacological mechanisms of drug improving the renal function. Methods： The healthy adult SD rats were operated with 5/6 nephrectomy,in addition to the sham -operated group, the 60 animals with chronic renal insufficiency and azotemia were randomly divided into model group, Niaoduqing group, Dengzhanxixin high -dose group, Dengzhanxixin middle dose group and Dengzhanxixin low - dose group , animal number of each group was 12. Dynamic observation of blood urea nitrogen and serum creatinine level in 4 weeks ,8 weeks and 12 weeks were done, when in 12 weeks renal tis- sue TNF-α content were measured using ELISA method , spectrophotometry for quantitative measurement of SOD, and statistical software SASS. 2e. Results ： There was some improvement in the dose group of Dengzhanxixin injection on renal insufficiency, the most obvious in the high- dose group, showing a dose -effect relationship, but weaker than Niaoduqing role （P 〈 0.05 ）. After Dengzhanxixin treatment TNF - -α level decreased and SOD content increased （ P 〈 0.05 ）. Conclusion：To sum up, the drug can improve chronic renal insufficiency ,its mechanism may be by reducing the level of TNF -α, promote froc radical scavenger SOD synthesis.