利心冲剂对心衰大鼠模型的治疗作用和凋亡相关基因的影响

Effect of Li Xin Granule on the Relate Gene of Apoptosis and Treatment of Rats Adriamycin-Induced Heart Failure

目的:探讨利心冲剂对心衰大鼠模型抗凋亡作用机制。方法:腹腔注射阿霉素(ADR)复制大鼠心衰模型,左心室插管术测定血流动力学指标,Real time PCR检测凋亡相关基因。结果:利心冲剂治疗组和模型组比较,左心室收缩内压显著升高;凋亡相关基因Bcl-2、Bcl-2/bax增高;均有显著性差异(P<0.05),利心冲剂治疗组心肌细胞变性坏死明显减轻。结论:利心冲剂提高凋亡相关基因Bcl-2、Bcl-2/bax表达,减轻心肌细胞凋亡,改善心脏收缩与舒张功能。

Objective： To observe the protective effect of Li xin granule on the relate gene of apeptosis of rats with adri- amyein - induced heart failure. Methods ： Sixty adult male Wistar rats were divided into 5 groups： normal control group, adriamycin（ADR） group ,L - Li x-in granule group, H - Li xin granule group, Captopril group. Then ADR of 5rag/kg was given intraperitoneally once to copy the model of heart failure once 5 days, Li xin granule of 2,4mL/100g/d was oral- ly administered through a stomach, tube once a day for 21days in L - Li xin granule group, H - Li xin granule group, Captopril group, respectively. The control groups were given the same amount of normal saline the same way. The biological signal collecting system was used to record and analyze the LVSP of the rats. The pathological changes of the cardiomyocytes were observed. Real time PCR was used to record and analyze the relate gene of apoptosis of rats. Result： As compared with NC group , the LVSP of the ADR group were significantly lower （P 〈0.05） , but those of the H - Li xin granule group were markedly higher than those of the ADR group （ P 〈 O. 01 ）. HE staining showed that the myocardial tissue in CHF group was injury, presenting in cardiomyopthy - like changes; n control group, muscle fiber was arranged regularly with no injury muscle fibers; intreatment group, Li xin granule partially normalized contractile function . Real time PCR and Serological detection staining showed that the relate gene of apoptosis （ Bcl - 2, Bcl - 2/bax） of rats. As compared with NC group , the LVSP of the ADR group were significantly lower （ P 〈 0.05 ） , but those of the H - Li xin granule group were markedly higher than those of the ADR group （ P 〈 0.01 ）. Conclusion： H - Li xin granule group treatment can relieve apoptosis of ADR on myocardium and also obviously improve the cardiac contractility of heart failure rats.