补肾中药对超促排卵小鼠着床期子宫内膜组织形态学的影响

Effect of Erzhitiangui Granules on the Endometrium Histomorphological Changes of Mice after Superovulation During Embryonic Implantation

目的:观察二至天癸颗粒对促排卵小鼠子宫内膜厚度及组织形态学指标的影响。方法:选用健康性成熟昆明种小鼠30只,随机分为治疗组(中药配合HMG组)、模型组(HMG组)和正常组(生理盐水组),于检栓第4天(D4)上午脱颈处死小鼠,宫角作石蜡包埋切片,行子宫内膜厚度及组织形态学指标测量。结果:模型组小鼠D4子宫内膜发育明显延迟,子宫内膜发育成熟率为30%,显著低于正常组,且内膜与间质发育可见不同步;治疗组内膜成熟率可达90%,与模型组比较,有极显著性差异,且治疗组子宫内膜腺体面积及腺腔面积与正常组接近,较模型组明显增加。结论:二至天癸颗粒可明显改善HMG促排卵周期子宫内膜组织形态学指标,提高子宫内膜成熟度,这可能是该方补肾益天癸改善子宫内膜容受性作用机制的一个方面。

Objective：To study the effect of Erzhi Tiangui Granules combined with ovarian stimulation on the endome- trium histomorphological changes of mice during embryonic implantation and the mechanism of TCM in improving endometrial receptivity. Methods ：Thirty mice were allocated into three groups randomly, （1）normal group （group NS） , performed abdomen injection with 0.9% salt solution of same volume as model group and study group on the same time schedule;（2 model group （group HMG ＋ HCG）, HMG and HCG were given for ovarian stimulation;（~）study group （group TCM ＋ HMG ＋ HCG） ： Erzhi Tiangui Granules combined with HMG and HCG were given. After cohabiting, vaginal plug was found on the second morning. It can be seen as pregnant 1 day （ Dt ）. On pregnant 4 days （ D4 ） , the samples of mice endometrium were eoUeeted, endometrial thickness ,the size of gland and glandular lumen were investigated by light microscope with computerized multi - functional image analyzer. Results： Comparing to the normal group, delayed endometrial maturation was frequently found in group of HMG ＋ HCG, the non - synchronistic development of gland epithelium and stroma cells, namely, pesudostratified glandular epithelium and predecidual changes of stroma cells were seen at same time in model group. Combined with Erzhi Tiangui Granules, the size of gland and glandular lumen were significantly larger than those in model group, and were similar to normal group. Conclusion：Therefore the protocol with HMG had a negative effect on endometrial histological structure, and Chinese medicine of tonifying kidney can improve the morphological index in mice endometrial tissue during implantation window under stimulation.