益气活血复方对动脉粥样硬化家兔纤溶系统及TF的影响

Effect of Replenishing Qi and Activating Blood Formula on PAI-1 t-PA TF with Arteriosclerosis in Rabbits

行颈动脉球囊损伤术及高脂饲料喂养造成家兔动脉粥样硬化模型,观察益气活血复方对动脉粥样硬化家兔组织型纤溶酶原激活物(t-PA)、组织型纤溶酶原激活物抑制剂-1(PAI-1)、组织因子(TF)及超微结构的影响。方法:40只雄性新西兰大耳白兔随机分成5组:正常组、模型组、中药对照组、西药对照组、中药治疗组,每组8只。适应性喂养1周后,模型组、中药对照组、西药对照组、中药治疗组行颈总动脉球囊损伤术。术后正常组给予普通饲料,其他组给予高脂饲料喂养4周。实验第4周末开始分别予与益气活血复方、血脂康胶囊和辛伐他汀片,持续给药4周。在治疗前、后分别取血检测PAI-1、t-PA、TF。8周末处死动物,取家兔左右颈总动脉,用透射电镜观察颈总动脉的超微结构。结果:模型组PAI-1水平持续升高,t-PA略有升高,TF水平明显升高。中药治疗组与模型组相比较,PAI-1水平明显下降,差异显著(P<0.01),t-PA水平明显升高,差异明显(P<0.01),TF水平明显下降,差异显著(P<0.01);电镜水平证实益气活血中药能够改善颈内动脉的超微结构。结论:益气活血复方能促进动脉粥样硬化家兔纤溶酶原激活物的分泌,减少纤溶酶原激活物抑制物的分泌,同时抑制组织因子释放,从而减少动脉粥样硬化的发生。

Objective： Establishing a model by injurying carotid arteries in cholesteroled rabbits. To explore the effects of replenishing Qi and activating blood formula（RAF） on PAI - 1, t -PA, TF with Arteriosclerosis in rabbits. Methods： Rabits randomly received high fatty feedstuff, simvastatin, xuezhikang eapsu/e or RAF beg/truing at 4 weeks post injur- ying carotid arteries for 4 weeks per gavage. PAI- 1, t - PA,TF expressionsat were detected by ELISA. Results ： Compare with the control group, the level of t -PA in the therapy groups was significantly increased（ P 〈0.01） ,and the level of PAI- 1 ,TF were reduced（ P 〈 0.01 ）. Conclusion ： Theses results demonstrated that RAF may prevent and treat arterio- sclerosis by enhance fibrinolytic functionreducing.