消可宁颗粒减少ONOO^-形成 保护高糖条件下内皮细胞的实验研究

Effects of Xiaokening in Different Concentrations on iNOS NO and ONOO^- Produced by Human Umbilical Vein Endothelial Cell

目的:观察体外高糖培养条件下,消可宁颗粒对血管内皮细胞内皮型一氧化氮合酶(endothelial nitric oxide synthase,eNOS)、诱导型一氧化氮合酶(induce nitric oxide synthase,iNOS)、一氧化氮(nitric oxide,NO)及过氧亚硝酸阴离子(peroxynitrite,ONOO-)的影响。方法:体外培养人脐静脉内皮细胞株ECV-304,分常规培养组、高糖对照组和不同浓度消可宁组,检测NOS和NO的吸光度,计算出活力或含量。用免疫组织化学方法检测ONOO-生成的标记物一硝基酪氨酸(nitrotyrosine,NT)的表达。结果与高糖对照组比较,消可宁组eNOS活力明显增加,iNOS活力降低,NO含量下降,NT表达减少。并具有剂量与时间依赖性。结论:消可宁可以抑制iNOS活性,并降低NO含量,减少血管内皮细胞内ONOO-形成而发挥抗氧化作用。

Objective：To explore the effects of Xiaokening in high glucose on nitric oxide synthase （NOS）, nitric oxide （NO） and peroxynitrite （ONOO-） produced by vein endothelial cells. Methods：Human umbilical vein endothelial cells strain ECV -304 were divided into seven groups：normal groups：high -glucose group （30mM） and the Xiaokening groups （20,40,60,80,100g/L）. The eNOS, iNOS, NO and nitrotyrosine （NT） were detected at the end of the experiment. Results ： Compared with high - glucose group, eNOS activity increased in Xiaokening groups, （ P 〈 0.05 ）. The change in 100g/L was greater than that in 20g/L （P 〈 0.05）. While iNOS, NO and NT decreased. Conclusion： Xiaokening can take more effect on inducing eNOS and inhibiting iNOS,NO and ONOO - release from vein endothelial cells than that of high glucose.