Chemerin基因mRNA在小鼠体内的表达

Chemerin gene mRNA expression in mice

目的:观察Chemerin基因mRNA在小鼠体内脏器及在3T3-L1脂肪前体细胞诱导分化过程中的表达情况。方法:体外培养3T3-L1脂肪前体细胞,诱导分化过程中收集9个不同时间点的细胞;同时取正常小鼠7种脏器,一并提取脏器组织和细胞中总RNA,半定量RT-PCR检测其中Chemerin基因mRNA表达水平。结果:Chemerin在脂肪组织、肝脏和肾脏为高;在3T3-L分化的过程中Chemerin基因表达量显著上调。结论:Chemerin是一种新的脂肪因子,参与脂肪细胞的分化,可能参与肥胖等代谢性疾病的发生。

Objective ： To observe the change of Chemerin gene mRNA expression in various tissues of mice and in 3T3-L1 preadipoeyte differentiation. Methods： Culture of 3T3-L1 cells and induction of differentiation were performed in vitro. Total RNA was extracted from seven kinds of tissues of mice and the differential cells at nine time points. Semi-quantitative RT-PCR was performed in order to measure levels of Chemerin mRNA. Results： Chemerin mRNA was expressed most highly in adipose tissue, liver and kidney. Chemerin mRNA expression dramatically increased during the process of differentiation of 3T3-L1 cells. Conclusions： Chemerin is a novel adipokine that participates in preadipocyte differentiation which indicates that it may play a key role in the development of obesity and other metabolic diseases.