摘要
将NDVD10毒株于鸭胚尿囊腔和绒毛膜两个途径接种传代至50代,分别测定不同代次胚液病毒HA、EID50及免疫原性,结果表明该病毒用尿囊腔途径接种也可获得较高滴度病毒,不同代次病毒液HA和EID50都分别在1∶256和108.0/01ml以上,免疫原性良好,但鸭胚致死性随传代次数增加而减轻,120小时内死亡率仅说明该病毒稳定性良好。该病毒于-20℃和-50℃保存期分别达6和12个月。
The study was made on the title and immunology of Newcastle disease virus,strain D 10 ,after 50 propagations in duck embryo inoculated by chouiolallantoic membrane and allantois.The results showed that The virus could propagate in duck embryo with high title,when it was inoculated by allantois.The HA value and EID 50 of the virus after various propagation were always more than 1∶256 and 10 8.0 /0.1ml,respectively.They minimum dose of immunity was less than 5000EID 50 .The title of this virus,stored at -20℃ and -50℃ for 6 and 12 months respectively,was hardly different.
关键词
新城疫病毒
D10株
免疫原性
鸡
Newcastle disease virus Strain D 10 Stbility immunology