摘要
设定了－１０，３，１５，２５，３５℃和自然温度６个水平贮藏东北红豆杉花粉．在贮藏期间采用氯化２，３，５三苯基四唑（ＴＴＣ）染色法每隔３ｄ测定１次花粉生活力．测定结果表明，东北红豆杉花粉寿命因贮藏温度不同而存在显著差异，温度越高花粉寿命越短．在自然温度下贮藏２周后，５０％东北红豆杉花粉便丧失了生活力，７周后全部丧失生活力．而在－１０℃冰柜中和３℃冰箱中分别贮藏４个月和２个月后，仍有２０％的花粉具有生活力．低温可延长东北红豆杉花粉的贮藏寿命．
Taxus' pollen was stocked in -10,3,15,25,35℃ and natural temperature tubers respectively.Pollen's vitality was identified by TTC every three days during stocked period.The results showed,Pollen's life span exists a considerable difference at different stocked temperature.Pollen's life span became shorter with the increasing of temperature.Fifty percent of Taxus' pollen lost vitality at natural temperature after two weeks,and no live pollen after seven weeks.And twenty percent of pollen has vitality at -10℃ after four months and at 3℃after two months respectively.Life span of Taxus will be prolonged at lower temperature.
