摘要
草鱼出血病病毒GCHV－892野毒株在PSF细胞连续传代过程，于培养液中加入一定浓度的桉液，传至19代已达减毒目的。继续传至29代其减毒效果保持稳定。27代后不再加入桉液，继续传至37代其减毒效果仍然保持稳定不变。致弱病毒对草鱼的安全性好，免疫原性强。用该弱毒株制备的弱毒疫苗免疫草鱼后，草鱼的成活率和免疫保护率均达100％。表明桉液是草鱼出血病病毒的一种良好减毒剂。经桉液减毒的GCHAV－892是一株良好的减毒株。
This paper describes that GCHV-892 has been domesticated continuously for 19 generations in cell 1ine (PSF) in culture solution with certain concentration of liquid being from An (Eucalyptus) leaves, and has been attenuated. Effect of attenuation has been stabilized for continuous domestication from 19 to 29 gen. The effect of attenuation has still been stabilized for continuous domestication up to 37 gen. After the An liquid did not add to the culture solution from 27~37 gen., there are good innocuity and strong immunogenicity of virus for grass carp. Attenuated live vaccine prepared with the attenuated strain for grass carp is 100% in innocuity survival rate and immune protection rate. It shows that the An liquid is a good attenuated medicine for virus of hemorrhage of grass carp, and GCHAV-892 attenuated with An liquid is a good attenuated strain.
出处
《上海水产大学学报》
CSCD
1998年第3期211-216,共6页
Journal of Shanghai Fisheries University
关键词
草鱼
出血病病毒
桉液
减毒
Ctenopharyngodon idella, virus of hemorrhage, An (Eucalyptus) liquid,attenuation
