应用AP-PCR技术检测香菇栽培菌株的遗传差异

Detection of Genetic Diversity of Cultivated Strains in Lentinula edodes by AP-PCR

利用3个随机引物对21个香菇栽培菌株进行PCR扩增。结果表明，3个引物共产生45条DNA谱带，其中分子量最小的为0．18kb，最大的为1．16kb，聚类分析表明，21个香菇菌株的相似系数为0．64～1．00，其中大部分菌株遗传差异较小而相似程度较高。

Three arbitrary primers were used to finger-print twenty - one cultivated strains of Lentinula edodes in this paper. The results revealed that three primers produced forty five DNA bands ranging from 0. 18 ～1. 16 kb in these strains. Cluster analysts indicated that the similarity coeffi-cients were 0.64～1.00, low genetic diversity and high similarity existed in most of these strains.