安徽黄山地区中华硬蜱及其宿主感染莱姆病Borrelia afzelii的检测

Natural Infection of Borrelia afzelii in Ixodes sinensis and Its Parasitism Hosts in Forest Areas of Huangshan, Anhui Province

利用PCR和病原体分离技术对安徽黄山地区中华硬蜱及其宿主感染莱姆病螺旋体的状况进行了检测，结果从40只黄山林区的中华硬蜱中获得了一株莱姆病螺旋体分离株，寄主动物及其他中华硬蜱均未获得阳性分离结果。以16srRNA为靶标进行PCR检测，6．78％雌性和5％雄性中华硬蜱发现有阳性感染，2只草兔（92只）和1只麂子（16只）也发现有阳性感染；若蜱和其余宿主动物未发现阳性感染。阳性分离株和扩增获得的16srRNA序列结果一致，同GenBank中的B．afielii的系统发育关系最近，提示该分离株螺旋体属于B．afzelii。该结果与前期的实验传播结果提示中华硬蜱是我国南方莱姆病的传播媒介。

A survey was conducted on Ixodes sinensis and its parasitism hosts for the infection of Borrelia burgdorferi with PCR and cultivation method. Only one spirochete strain was isolated from 40 1. sinensis collected in Huangshan Mountains. While all blood sample and other I. sinensis ticks fail to get Lyme spirochetes isolation. When detected by 16s rRNA amplification, 6.78 % female and 5% male adult I. sinensis ticks yield positive result, two of 92 Chinese hares and one of 16 Chinese muntjacs also appear to be positive with the same DNA band, while all nymph ticks appeared uninfected. However, these DNA amplicon have the same 16S rRNA sequences without any difference. The isolated spirochetes strain obtained from I. sinensis in Huangshan show high similarity to other strains of B. afzelii by phylogeny analysis with 16S rRNA sequences and might belong to Borrelia afzelii species. The finding together with the proved transmission ability, imply that I. sinensis might be the vector tick species in southern China.