冠心病患者肺炎衣原体包涵体的检测和意义

Detection of Chlamydia pneumoniae inclusion body in patients with coronary heart disease

目的:探讨冠心病(CHD)患者血液肺炎衣原体(Cpn)包涵体的检测和临床意义。方法:收集2006年CHD患者150例和同期体检正常者55例的血液标本,采用-85℃冰冻保存的外周抗凝血液,低渗溶血制备淋巴细胞悬液,用直接免疫荧光法(DIF)检测特异性Cpn包涵体。结果:冠心病组150例,包涵体阳性20例,阳性率为13.3%;健康对照组55例,包涵体阳性1例,阳性率为1.8%,差异有统计学意义(P<0.001),相对危险度为8.3,95%的可信区间为1.09～63.46。结论:CHD患者Cpn包涵体阳性率高于健康者,说明CHD患者存在Cpn感染状况。

Objective： To explore the value of Chlamydia pneumonia （Cpn） inclusion body detection in peripheral blood mononuclear cell （PBMC） of patients with coronary heart disease （CHD）. Methods： The anticoagulant peripheral blood of CHD patients were preserved at -85℃. and lymphocyte suspension were prepared with hypoosmotic hemolysis. Cpn inclusion body was detected by direct immunofluorescence （DIF）. Results： Cpn inclusion bodies was detected in 20 （13.3%） of 150 patients with CHD and in 1 （1.8%）of 55 in healthy control group. The positive rate of inclusion bodies in CHD group was significant higher than those in control group （P 〈 0. 001 ）. Conclusion ： Patients with CHD have higher showing evidence for the presence of Cpn inclusion bodies and was infecting