微波萃取-气相色谱/质谱联用法测定大蒜中大蒜新素

Determination of Diallytrisulfide in Garlic by Gas Chromatogrphy/Mass Spectrometry with Microwave Assisted Exteraction

建立了用微波萃取技术提取大蒜中的大蒜油的方法,并对提取物中的大蒜新素进行气相色谱-质谱分析,用外标法测定其含量。通过对萃取条件的选择,得出微波萃取大蒜油的最佳条件选二氯甲烷为萃取剂,萃取剂用量为200 mL,萃取时间为30 s。实验结果表明利用微波萃取技术对样品处理以及使用GC/MS法测试大蒜中大蒜新素,该方法具有快速、萃取剂用量少、精度好、回收率高、准确可靠等优点。

A method has been developed for analysis of diallytrisulfide in garlic by GC/MS with microwave extraction using external standard method.The optimized extraction conditions are as follows： solvent,volume of solvent and extraction time are dichloromethane,200 mL and 60 sec respectively.This methods is of simple,fast,less solvent,accurate,higher recovery,so is suitable to the determination of content of diallytrisulfide in garlic.