eNOS和P450在大鼠性成熟期睾丸的表达及意义

Expressions of eNOS and Cytochrome P450 in the Testis of Sexually Mature SD Rats and Their Significance

目的:探讨内皮型一氧化氮合酶(eNOS)和细胞色素P450(芳香化酶)在雄性SD大鼠性成熟期睾丸中的表达及意义。方法:选取出生后5周、7周和10周的雄性SD大鼠各6只,左侧睾丸行石蜡切片,运用免疫组化ABC法观察eNOS和P450在雄性SD大鼠性成熟期睾丸中的表达变化情况。结果:出生后5周、7周和10周,睾丸间质细胞胞质eNOS和P450免疫反应阳性,阳性程度皆为(+++)、(+)和(++);精母细胞胞质eNOS和P450免疫反应阳性表达,但细胞数量较少,且无规律可循。结论:在性成熟期雄性SD大鼠的睾丸间质细胞中,随着性成熟发育的进展过程,eNOS和P450的表达强弱有所不同,且两者具有关联性。

Objective： To explore the expressions of endothelial nitric oxide synthase（eNOS） and cytochrome P450（aromatase） in the testis of sexually mature male SD rats and their significance.Methods： Eighteen male SD rats,6 five-week,6 seven-week and 6 ten-week old,were selected for this study.Paraffin sections of the left testis were made and the expressions of eNOS and P450 observed by the immunohistochemical ABC method.Results： Positive expressions of eNOS and P450 were found to be ＋＋＋,＋ and ＋＋ in the Leydig cells of the five-week,seven-week and ten-week old rats,respectively,and they were also observed in a few spermatocytes,though with no regularity.Conclusion： In the Leydig cells of sexually mature male SD rats,eNOS and P450 are differently expressed in different stages of sexual maturation,and they are correlated as well.