摘要
目的研究越橘总黄酮对人宫颈癌细胞株Hela细胞中TRAIL及其受体表达的影响。方法用浓度为0、0．025、0．25、2．5、和25μg／ml的越橘总黄酮处理Hela细胞24h后，采用四甲基偶氮唑盐（MTT）方法检测越橘总黄酮对Hela细胞增殖的抑制作用，采用Hoechst33342／PI染色观察越橘总黄酮对Hela细胞凋亡的诱导作用，采用RT—PCR法检测不同浓度越橘总黄酮处理后的Hela细胞中TRAIL及其受体mRNA基因表达变化。结果0．025、0．25、2．5、和25μg／ml的越橘总黄酮对Hela细胞增殖的抑制率分别为22．08％、36．04％、42．10％和44．70％；0．25～25μg/ml的越橘总黄酮处理组细胞呈现明显的凋亡形态学改变；TRAIL和TRAIL—R2表达量明显高于空白对照组（P〈0．05），TRAIL—R3和TRAIL—R4表达量明显低于空白对照组（P〈0．05），而TRAIL—R1在各处理组间差异无统计学意义（P〉0．05）。结论越橘总黄酮诱导Hela细胞凋亡的机制可能与上调TRAIL及其受体TRAIL—R2基因的表达有关。
Objective To study the effect of bilberry flavones on the expression of RAIL and it＇s receptor in Hela cell lines. Methods Proliferation of Hela cells was measured by MTT assay after exposure to bilberry flavones at the concentrations from 0. 025 to 25 μg/ml for 24 hours. Morphology of cell apoptosis was observed by Hoechst33342/PI fluorescent staining. The mRNA expression of apoptosis-related genes TRAIL and TRAIL-R of Hela were determined by RT-PCR. Results Inhibition rate of different Bilberry flavones concentration from 0. 025,0. 25,2.5 to 25μg/ml were 22. 08% ,36. 04% ,42. 10% and 44. 70% , respectively. Hela ceils showed significant apoptotic morphology changes after exposure to bilberry flavones at concentrations from 0. 25 to 25 μg/ml for 24 hours. Expression of TRAIL and TRAIL-R2 was significantly higher than that in control group （ P 〈 0. 05 ）. Expression of TRAIL-R3和 TRAIL-R4 was significantly lower than that in control group （ P 〈 0. 05 ）. TRAIL-R1 expression had no significant difference （ P 〉 0.05 ）. Conclusion Up-regnlation of TRAIL and TRAIL-R2 gene expression by bilberry flavones might be involved in the induction of apoptosis in Hela cells.
出处
《中国医师杂志》
CAS
2009年第10期1297-1301,共5页
Journal of Chinese Physician
基金
基金项目：黑龙江省人事厅博士后基金（LBH-Z06252）
