大鼠急性脊髓损伤后引起肺损伤肺中NMDA受体的表达及意义

The NMDA receptor expression and significance in the pulmonary after acute spinal cord injury causing lung injury in rats

目的探讨大鼠急性SCI后引起肺损伤肺组织中NMDA受体的表达及其意义。方法36只Wister大鼠SCI造模后分为模型组（A组）和MK-801处理组（B组），在12h、1d、3d 3个时间点（各6只）处死大鼠，B组动物均于处死前6h腹腔给予0．1mg／kg MK-801。另设6只为对照组，取肺组织测W／D、肺泡灌洗液中细胞和蛋白漏出量，并观察肺显微形态的变化。结果病理结果示A组大鼠伤后12h即有出血、水肿，伤后3d达到高峰，MK-801可以改善上述情况且可明显减轻SCI后W／D、红、白细胞记数及蛋白含量的升高（P〈0．05）。结论大鼠急性SCI后早期出现肺组织出血和水肿，这可能是早期呼吸功能衰竭和肺部感染的病理基础，并可能以激活上调NMDA受体而引起急性肺损伤。

Objective To investigate the NMDA receptor expression and significance in the lung injury caused by acute spinal cord injury. Methods 36 rats were divided into two groups： model group （group A） and MK-801 treated group （group B） which were divided into subgroups of 12h, 1, 3 days after injury respectively（ each subgroup, n = 6）. All animals were sacrificed at the time points （12h, 1 d, 3d） after injury. Croup B were treated with 0. 1 mg/kg MK-801 6h before sacrificed. The lung W/D, the content of cells and the total protein in bronchoalveolar lavage fluid （BALF） were determined. Results In group A, pulmonary hemorrhage and edema were observed at the 12th hour, and reached peak at the 3rd day after injury. Those parameters determined in group B were significantly improved than that in group A （ P 〈 0. 05 ）. Conclusions Pulmonary hemorrhage and edema appear in the early stage after spinal cord injury in rats, which may be the pathological basic of the early respiratory failure and pulmonary infection, and it may induce the acute lung injury through the activation of NMDA receptor.