功能近红外光谱对激光诱导间质热疗法治疗肝癌实时在位疗效评估的初步研究

Fundamental research of functional near infrared spectroscopy of hepatic tumor in laser induced interstitial thermotherapy in real time

目的:探讨功能近红外光谱(fNIRS)实时在位监测肝癌激光热疗的敏感性、可行性。方法:体外培养H22肝癌细胞,建立小鼠的肝脏原位移植肿瘤,分不同时间相同功率及不同功率相同时间组分别对肝肿瘤进行激光热疗,同时利用fNIRS监测系统对热疗过程进行实时监测获取相关参数,术后第1天利用7.0 T核磁共振(MRI)及病理学检查确定肿瘤毁损范围。结果:以病理学结果为激光疗效判断标准,在相同时间不同功率组MRI在2.1 W未提示损伤,fNIRS监测系统获取的优化散射系数(μs)出现稳定的平台期;相同功率不同时间组MRI一直未提示组织损伤,而μs在150 s出现稳定平台期。结论:fNIRS相对目前主要的肿瘤热疗监测手段MRI更加敏感,为开发实时在位激光诱导肿瘤间质热疗精确治疗系统打下基础。

Objective To explore the sensitiveness and utility of functional near infrared spectroscopy （fNIRS） in laser induced interstitial thermotherapy（LIIT） in real time. Methods The hepatic carcinoma cells H22 were cultured in vitro, and situ hepatic cancer models in mice were established. The mice were divided into two groups. One was labeled in different time and same watt, another was labeled in different watt and same time. fNIRS was done in real time while taking LIlT in the mice. The destructive extent was checked by MRI and pathological section in the first day after operation. Results In the group of the same time, MRI didn＇t reflect damage at the 2.1 W level, but reducing scattering oefficient （μs） appeared a steady plateau. In the group of the same watt, MRI didn＇t reflect damage all the time, but μs appeared a steady plateau at 150 s. Conclusion fNIRS is more sensitive than MRI, used more often currently. And that is the fundament to construct the precise assessing system in real time in LIIT.