丹参酮对血管性痴呆模型大鼠学习记忆障碍的改善作用

Effectiveness of Tanshinone on learning and memory impairment of vascular dementia model rats

目的:研究丹参酮对拟人类血管性痴呆(VD)模型大鼠学习记忆功能障碍的改善作用。方法:采用改良的大脑中动脉栓塞(MCAO)致局灶性脑缺血(3 h)和再灌注(30 min)法,建立VD样学习记忆障碍的大鼠模型。实验动物随机分成VD模型组、丹参酮治疗组、假造模对照组和正常对照组等4组。采用定位航行和空间探索试验测定大鼠的空间学习记忆功能。结果:(1)VD大鼠定位航行试验的逃避潜伏期明显延长;空间探索试验的逃避潜伏期延长更明显,而逃避频率降低,与两对照组比较有显著性差异(P<0.01)。(2)丹参酮治疗后,VD大鼠定位航行试验的逃避潜伏期缩短;空间探索试验的逃避潜伏期缩短更明显,而其安全逃避频率有明显提高。结论:(1)本研究制成的VD样学习记忆障碍的动物模型具有较好仿真人类VD的特点;水迷宫试验能较真实地反映动物学习记忆功能。(2)丹参酮对VD样大鼠的学习记忆功能障碍具有一定的改善作用。

Objective To study the improvement of learning and memory impairment in rat models of vascular dementia （VD） with Tanshinone. Methods Rat VD model was established by modified middle cerebral artery occlusion method. The experimental animals were randomly devided into four groups： VD model group, Tansbinone-treated group, false model group and normal control group. Learning and memory function of rats was assessed by place navigation and spatial probe. Results （ 1 ） Escape latency of VD rats was obviously prolonged, and frequency of escape was decreased significantly as compared with two control groups. （2） After Tanshinone-treated group was treated with Tanshinone, escape latency of VD rats was obviously shortened,and frequency of escape was obviously increased. Conclusions （ 1 ） VD- like animal models of learning and memory impairment have characters of VD. A water- maze test can accurately assess learning and memory function of rats. （2） Tanshinone can improve the learning and memory impairment of VD-like rats.