摘要
目的:了解OY-TES-1mRNA及其蛋白在肿瘤组织中的表达情况,初步探讨其结构与功能。方法:利用定量PCR和免疫组织化学技术检测肿瘤组织中OY—TES-1的表达,结合生物信息学技术分析其结构和功能。结果:肿瘤与瘤旁组织中目的基因mRNA的表达频率分别为61.95%和59.57%,其中肝细胞癌72.97%、脑膜瘤55.5s%、胶质瘤57.50%,肿瘤组织中该基因mRNA的表达量明显高于瘤旁组织。目的蛋白在胃癌的阳性率为25.00%,肝细胞癌40.00%,结肠癌46.67%,而瘤旁组织与肺癌均为阴性反应。生物信息学分析提示目的蛋白富含螺旋结构,具有一个信号肽、多种修饰位点及sp32结构域,存在较多T、B细胞表位且主要位于目的蛋白的羧基端。结论:OY-TES-1mRNA及其蛋白均可在肿瘤组织中表达,生物信息学分析结果提示该蛋白功能的多样性。
Objective: To explore gene expression characteristics of OY-TES-1 mRNA and protein level in tumor tissues, and predict its structure and function by bioinformatics analysis. Methods: Tumor tissues were tested to explore the expression of OY-TES-1 mRNA and protein by quantitative PCR and immunohistochemistry staining. The structure and function of OY TES-1 were predicted by bioinformatics techniques. Results: The expressive frequencies of OY-TES-1 mRNA in tumor and adjacent tumor tissues were 61.95% and 59.57% respectively, including 72. 97% in hepatocellular carcinoma, 55.56% in meningioma and 57.50% in glioma, and the expression level of OY-TES-1 mRNA in tumor tissues was higher than that in adjacent tumor tissues. The expressive rate of OY-TES-1 protein in tumor tissues was 25.00% in gastric carcinoma, 40. 00% in hepatocellular carcinoma, and 46.67% in colonma tissues, while adjacent tumor tissues and lung cancer showed negative. Bioinformatics method indicated that target protein was full of helices with a signal peptide, multitude modit'ication sites and sp32 domains. Immunogenicity of OY-TES-1 protein was found with many T cell epitopes and B cell epitopes, which mainly exist in carhoxyl terminus of the target protein. Conclusion: Both OY-TES-1 mRNA and protein could be found in tumor tissues, and the functional diversity of this protein can be predicted by bioinformatics techniques.
出处
《解剖学杂志》
CAS
CSCD
北大核心
2009年第5期603-606,共4页
Chinese Journal of Anatomy
基金
国家自然科学基金(30360026,30760055)
广西科学基金(桂科自0728148)