麇鹿角醇提液对雄鼠性腺功能的影响

Effect of Elk antlers ethanolic fluid extract on gonad function of male mice

目的：观察麋鹿角醇提液对雄性小鼠性腺功能的影响，对麋鹿角传统的壮阳作用进行初步实验研究。方法：以重复电刺激复制应激小鼠性功能低下模型，观察麇鹿角醇提液对成熟雄鼠捕捉雌性小鼠能力的影响。给正常幼龄雄性小鼠连续灌胃给药10d，观察其性器官以及附性器官的脏器质量指数，检测其精子密度和精子活动率，并测定血清中睾酮与促黄体生成素的水平。结果：麋鹿角醇提液能增加应激后雄鼠捕捉雌鼠的能力，能够缩短跨骑潜伏期，增加舔舐和跨骑次数。麇鹿角醇提液能增加幼龄雄性小鼠睾丸质量指数，但对其附性器官质量指数没有影响，对其精子密度与精子活动率也没有影响。能提高幼鼠促黄体生成素水平，但对睾酮的水平没有影响。结论：麋鹿角醇提液对电刺激应激诱发的雄性小鼠性功能障碍有促进恢复作用；麇鹿角醇提液具有一定促性激素样作用，可能不具有雄性激素样作用。

Objective： To investigate the effect of Elk antlers ethanolic fluid extract on gonad function of male mice and make a preliminary research on traditional pharmacological actions of Elk antlers strengthening sexual ability. Methods： Repeated electrical stimulation stress was used to copy sexual dysfunction model mice. The influence of Elk antlers ethanolic fluid extract on mature male mice＇s ability of capturing female mice was observed. Elk antlers ethanolie fluid extract was given to immature male mice for 10 days, then the weight index of sex organ and accessory sex organ were observed, sperm density and sperm activity were detected, and the levels of testosterone and luteinizing hormone in serum were determined. Results： Elk antlers ethanolic fluid extract could strengthen the sexual ability of stressed mature male mice in capturing female mice. It decreased latency of licking and mounting behavior, and increased the frequency of the licking and mounting behavior. It raised the mass index of testicle in inmature male mice, but had no effect on the weight index of accessory sex organ and the density and activity of sperm. It elevated the level of luteinizing hormone but not testosterone. Conclusion： Elk antlers ethanolic fluid extract has a protective effect on the sexual dysfunction induced by electrical stimulation stress; and it has certain gonadorelin-like function but may not have sex hormone-like function.