来曲唑诱导大鼠多囊卵巢综合征模型卵巢组织中CYP19基因的表达

Expression of CYP19 gene in ovary tissue of rat model of letrozole-induced polycystic ovary syndrome

目的：研究来曲唑诱导的多囊卵巢综合征（PCOS）模型大鼠卵巢组织中CYP19基因的表达，为该模型的应用提供一定的理论依据。方法：SD大鼠随机分为模型组和对照组，模型组应用来曲唑诱导大鼠PCOS模型。放射免疫法测定大鼠血清性激素水平；H～E染色观察卵巢组织学变化，免疫组织化学、Realtime-PCR和免疫印迹法检测CYP19基因在卵巢组织中的表达。结果：模型组血清性激素水平睾酮、黄体生成素、卵泡刺激素浓度显著增高，雌二醇、孕酮浓度降低。模型组CYP19mRNA及蛋白表达均显著高于对照组。结论：来曲唑诱导的PCOS大鼠卵巢组织中CYP19基因表达水平增高。

Objective： To investigate CYP19 mRNA and protein expression in ovary tissue of rat model of letrozole-induced polycystic ovary syndrome （PCOS）, and to provide experimental data for the model application. Methods： Forty rats were divided into two groups randomly, PCOS group administered letrozole once daily during 21 and a control group. Gonadal hormone concentrations in serum were determined by radioimmunoassay, the histologie changes in ovaries were observed by H-E staining, and the expression of CYP19 gene in ovary tissue was detected by Real time-PCR, Western blot and immunohistochemistry. Results： Compared to the control group, estradiol and progesterone showed a considerable reduction （P〈 0. 05）. Levels of serum testosterone, FSH and LH in the model group were markedly increased than that in the control group （P%0.05）. The expressions of CYP19 mRNA and protein were significantly up-regulated in the model group than those in the control group. Conclusion： The expression of CYP19 gene in letrozole-induced polyeystic ovary is significantly higher than that in the control ovary.