人精液一氧化氮含量与男性不育的相关性

Correlation of concentration of nitric oxide in male semen with male infertility

目的：研究精液中一氧化氮（NO）含量对精子凋亡的影响及其与男性不育之间的相关性。方法：采用精子质量检测系统对精液标本进行常规检查；应用硝酸还原酶法测定精液中NO的含量；应用瑞姬染色和TdT介导的原位末端标记（TUNEL）法检测凋亡精子；观察凋亡精子的形态结构改变。结果：不育组精液中NO（58．37±14．14）μmol／1比正常生育组精液中NO（35．20±8．23）／μmol／1含量高；正常生育组精子凋亡率9．67±2．54％比不育组精子凋亡率33．98％±10．54％低。结论：不育组精液中精子的凋亡率随着NO含量的增高而增加。高浓度的NO导致精子凋亡率增加而致使男性生育力下降。

Objective： To explore correlation of concentration of nitric oxide （NO） with sperm apoptosis rate as well as male infertility. Methods： Semen routine examination was done by sperm quality detection system; and NO concentration was tested by nitric oxide reductase. Apoptosis of sperm was examined by terminal deoxynucleotidyl transferase mediated deoxyuridine triphophate-biotin nick end labeling （TUNEL）. The ultrastructure of apopto6c sperm was observed under an electron microscope. Results： The NO concentration in semen was （58. 37±14.14）μmol/1 in the male infertility group and （35.20±8.23）/μmol/1 in the control group. The apoptosis rate of sperm was （9.67±2.54）% in the control group, while that of the male infertile group was （33.98±10. 54）%. The NO concentration and the apoptosis rate of sperm in the male infertile group was higher than that in the control group （P〈0.05）. Conclusion： Abnormal high level of NO in semen causes the increasing rate of sperm apoptosis, which may be related to male inferticity.