摘要
目的:观察人食管上皮细胞在聚乳酸-聚乙醇酸(PLGA)支架上的贴附和生长情况,利用组织工程技术培养工程化人工食管。方法:制作PLGA三维细胞支架;分离培养成人食管上皮细胞,体外扩增后种植到PLGA支架上。在体外和裸鼠体内分别培养食管上皮细胞-支架复合物,分期终止培养,进行组织学染色、扫描电镜、细胞角蛋白免疫组织化学检测。结果:体外培养显示,人食管上皮细胞在支架材料上贴附生长良好,长期培养仍保持食管上皮细胞特性,裸鼠体内培养4周后可形成食管黏膜样组织。结论:PLGA支架适合食管上皮细胞黏附生长,可作为食管组织工程的细胞载体。
Objective: To verify the adhesion and growth ability of human esophageal epithelial cells (HEECs) on poly-lacticco-glycolic acid (PLGA), a three-dimensional biodegradable polymer scaffold, and to reconstruct human esophagus by tissue engineering. Methods: HEECs isolated from adult underwent esophagectomy were seeded onto PLGA scaffold after expansion by in vitro culture. The complex of cell-scaffold was then cultured ex vivo and transplanted to subcutaneous of nude mouse respectively. The cell-seeded scaffolds in different culture intervals were assessed by H-E staining. Scanning electron microscopy was utilized to verify the interactions between the cells and the biomaterial. Immunohistochemical analysis was also performed to evaluate cytokeratin. Results: HEECs were well distributed and adhered to PLGA scaffolds and maintained their characteristics throughout the culture period. Both the first passaged esophageal epithelial cells and seeded cells could express and product cytokeratin. After being cultured in vivo for 4 weeks the cell-seeded scaffolds grew like tissues. Conclusion: PLGA scaffolds can support the esophageal epithelial cells proliferation.
出处
《解剖学杂志》
CAS
CSCD
北大核心
2009年第5期669-672,共4页
Chinese Journal of Anatomy
基金
福建省青年科技人才创新项目(2004J066)
国家自然科学基金(30271293)
关键词
食管
上皮细胞
组织工程
聚乳酸-聚乙醇酸
esophagus
epithelial cell
tissue engineering
poly-lactic-co-glycolie acid