除草剂西玛津对小鼠的免疫毒性作用

Immunotoxic effect of herbicide simazine exposure in BALB/c mice

目的探讨均三氮苯类除草剂西玛津对小鼠的免疫毒性。方法BALB／c小鼠30只，按体重随机分为5组，每组6只，分别为西玛津90、200、400mg／kg染毒剂量组（每日1次，连续21d灌胃染毒），阴性对照组（予蒸馏水）和阳性对照组（腹腔注射20mg／kg环磷酰胺）。观察小鼠体重变化、脾脏和胸腺的脏器系数、脾脏T细胞转化增殖能力以及血清中自细胞介素（IL）-2、IL-4、IgG和IgM含量。结果200与400mg／kg染毒组小鼠体重、脾脏和胸腺脏器系数以及T细胞转化增殖能力均明显低于阴性对照组，差异均有统计学意义（P〈0．05或P〈0．01）；200mg／kg染毒组小鼠血清中IL-2、IL-4、IgG和IgM含量分别为（108．50±3．20）pg／ml、（36．54±3．36）pg／ml、（46．25±7．41）ug／ml、（17．58±2．23）ug／ml；400mg／kg染毒组小鼠血清中IL-2、IL-4、IgG和IgM含量分别为（85．70±4．00）pg／ml、（35．92±2．29）pg／ml、（40．08±6．80）ug／ml、（11．92±3．23）ug／ml，均明显低于阴性对照组，差异均有统计学意义（P〈0．05或P〈0．01）。结论西玛津对小鼠细胞免疫和体液免疫功能均有抑制作用。

Objective To examine the effect of simazine on selected immune parameters in BALB/c mice. Methods Simazine （90, 200, 400 mg/kg） was administrered by oral gavage for 21 days in adult BALB/c mice. The negative control group unith distilled water and positive control group administered with cyclophosphamide in abdominal cavity were also established. After the last simazine dose, the mice were sacrificed, and blood, spleens, and thymuses were collected and processed for detection. The relative weight of spleen and thymus was calculated. The rate of T cell in spleen and the concentration of IL-2, IL-4,IgG and IgM were detected by ELISA. Results The weights of mice were decreased in 200 mg/kg and 400 mg/kg simazine groups. Thymus and spleen weights were decreased in 200 mg/kg and 400 mg/kg simazine groups compared with the negative control group. The concentration of IL-2, IL-4, IgG and IgM in serum of 200 mg/kg group were （108.50＋3.20） pg/ml, （36.54±3.36）pg/ml, （46.25±7.41） ug/ml, （17.58±2.23） ug/ml respectively ;The concentration of IL-2, IL-4, IgG and IgM in serum of 400 mg/kg group were （85.70±4.00） pg/ml,（35.92±2.29） pg/ml, （40.08±6.80） ug/ml, （11.92±3.23） ug/ml respectively （P〈0.05 or P〈0.01 ）. These results were decreased significantly compared with negative group. Conclusion Simaziue can inhibit the cellular immune function and the humoral immune function.