健脾清热化瘀中药对胃溃疡愈合质量影响的实验研究

Effect of Drugs of Invigorating Spleen,Heat-clearing and Eliminating Stasis on QOUH in the Rat Model of Gastric Ulcer

目的:探讨健脾清热化瘀中药对大鼠乙酸性胃溃疡愈合质量的影响。方法:采用冰醋酸注射法制备大鼠胃溃疡模型,随机分为模型组、雷尼替丁组(西药组)、健脾中药组、清热中药组、化瘀中药组、健脾清热化瘀中药组(合用组)和正常对照组(空白组)。于给药14 d后在光镜下观察溃疡指数(UI)、再生黏膜厚度、黏膜肌层缺损宽度及囊状扩张腺体数量,应用ELISA法检测血清表皮生长因子(EGF)及6-酮-前列腺素F1α(6-keto-PGF1α)含量,免疫组化法检测胃黏膜表皮生长因子受体(EGFR)的表达。结果:健脾、清热、化瘀中药及其合方都能使再生黏膜厚度增加,黏膜肌层缺损宽度及囊状扩张腺体数量减少,与模型组比较P<0.01,中药合方组疗效明显优于西药组(P<0.05或P<0.01)。健脾清热化瘀中药可明显提高血清6-keto-PGF1α含量,与模型组比较P<0.01,接近空白组水平,明显高于雷尼替丁组(P<0.05)。中药各组及西药组血清EGF含量较模型组及空白组升高(P<0.01),且可明显增强胃溃疡黏膜EGFR的表达(P<0.05或P<0.01),尤以中药合方组疗效最为明显(P<0.01)。结论:健脾清热化瘀中药通过改善胃溃疡再生黏膜组织学成熟度和功能成熟度,可明显改善实验性胃溃疡愈合质量,有效减少复发。

Objective： To study the effect of drugs of invigorating spleen, heat-clearing, eliminating stasis on QOUH in rat model of gastric ulcer. Methods：The rat model of gastric ulcer was constructed by injection of acetic acid. The rats were randomly divided into model group, ranitidine group, invigorating spleen group, heating-clearing group, eliminating stasis group, complex prescription group and normal control group. The ulcer index（UI）, regenerated mueosal thickness, lamina muscularis mucosal width and capsular broaden glandular organ quantity were observed by HE, and the levels of EGF and 6-keto-PGF1α were detected by ELISA. The expression of EGFR in the mucosa around the ulcer was detected by SABC kit immunohistochemical method. Results： Compared with Model group, the regenerated mucosal thickness, lamina muscularis mucosae defect extent, UI and capsular broaden glandular organ quantity in invigoraing spleen, heat-clearing, elimianting stasis and complex prescription treating groups were different significantly（ P 〈 0.01 ）. And the effect of complex prescription was better than ranitidine in different degree（ P 〈 0.05 ,or P 〈 0.01）. Compared with ranitidine, invigorating spleen group, heating-clearing group, eliminating stasis increased the levels of 6-keto-PGF1α closed to the normal. Compared with model group and normal control group, the levels of EGF in serum in treating groups were increased significantly （P 〈 0.01 ）. Positive expression of EGFR around ulcerative tissues in treating groups were strengthened obviously（ P 〈 0.05, or P 〈 0.01 ）, and the effect of complex prescription was the best（ P 〈 0.01）. Conclusion： Drugs of invigorating spleen, heat-clearing and eliminating stasis can improve the QOUH and prevent from recurring by improving histological maturation and functional maturation of regenerative mucosa in GU rats.