小承气汤证兔动物模型的研究

Study of the model of rabbits with the syndrome of Xiao Chengqi Tang

目的研究小承气汤证动物模型。方法将60只日本大耳白兔随机分为正常对照组、病理模型组、模型治疗组和模型验证组。在给予次碳酸铋、禁水的基础上,用适当剂量的大肠杆菌内毒素攻毒,观察家兔的体温、腹围、呼吸、二便等一般状态,检测相关的实验室指标。结果病理模型组家兔体温明显升高,腹围显著增大,实验指标与正常对照组比较差异有统计意义(P<0.01或P<0.05)。其中WBC、MDA和MMS含量有不同程度的增高,SOD含量明显下降;钾含量则显著降低;全血黏度升高,红细胞变形指数降低;TXB2、TM值和PAI-1含量明显升高,而t-PA含量明显降低。模型治疗组、模型验证组与病理模型组比较差异均有统计意义(P<0.01或P<0.05)。结论研制出的小承气汤证家兔病理模型,在症状、体征上符合传统中医理论的小承气汤证;在微观方面存在着热毒亢盛、气津两伤、瘀血内阻等病理变化。

Abstract：Objective To study animal model of the syndrome of Xiao Chengqi Tang. Methods 60 Japan rabbits of big ear were randomly divided into 4 groups. On base of giving bismuth subcarbonate and waterdeprivation, used proper dose of colitoxin to attack, and observed body temperature, abdominal perimeter,breathing, urination and defecation and so on, detected related experimental indexes. Results Body temperature and abdominal perimeter of rabbits in model group were obviously increase and enlarge, experimental in- dexes had obvious difference between model group and normal control group（P 〈0. 01 or P 〈0. 05 ）. The contents of WBC, MDA, MMS, TXB2, TM, PAI-I and whole blood viscosity were increase, the contents of SOD, kalium, t-PA and RBC ameboid index were obviously decrease. Compared with model group , treatment group and testing and verifying group had obvious difference （P 〈0. 01 or P 〈0. 05）. Conclusion The pathologic model of rabbits is in keeping with the syndrome of Xiao Chengqi Tang in symptom and sign, and has the pathologie changes of vigorous noxious heat, deficiency of both qi and yin, accumulation of blood stasis in microcosm.