螯合微粒共振散射光谱分析测定痕量钴

Resonance Scattering Determination of Trace Bobalt Based on Formation of Dithizone Complex Particles

研究了螯合微粒共振散射光谱的分析应用。结果表明，在pH为9．5的NH3-NH3Cl缓冲溶液中，Co（Ⅱ）与DDTC形成稳定的螯合物微粒体系，存在共振散射效应。在一定质量浓度范围内，Co（Ⅱ）的质量浓度与共振散射强度（IRS）呈较好的线性关系。其线性范围为0．012～1．44μg／mL，检出限为0．0012μg／mL，用于针剂维生素B12中钴的测定，方法简单、灵敏度高、重现性好，结果满意。

In pH 9. 5 NH3- NH4Cl buffer solution, Co（ Ⅱ ） and sodium diethyldithiocarbameate combine to stable complex particles, which exhibit resonance scattering effect at 470 nm. The scattering intensity at 470 nm is linear to Co（ Ⅱ ） concent ration in the range of 0. 012 - 1.44μg/mL. The detection limit is 0. 001 2 μg/mL Co. This method has been applied to determination of Co in vitamin B12, with sensitivity, simplicity, reproducibility and satisfactory results.