不同泌乳期小鼠乳腺乳铁蛋白基因的表达

Expression of Lactoferrin mRNA in Mice Mammary Gland at Different Lactation Stages

选取昆明小鼠为研究对象,以看家基因GAPDH为内参,用RT-PCR法对不同泌乳期小鼠乳腺组织乳铁蛋白基因的表达进行了研究。结果显示:不同泌乳期小鼠乳腺均有乳铁蛋白基因的表达,表达水平随泌乳过程的进行呈下降趋势,泌乳早期与中期和晚期差异显著。提示乳铁蛋白在泌乳期对小鼠乳腺免疫具有一定的调节作用。

To clarify the immune role of lactoferrin in mice mammary gland, half quantity RT- PCR was used to study the expression of lactoferrin mRNA in the mammary gland at different lactation stages of Kunming mice by taking the housekeeper gene GAPDH as a reference. The results showed that lactofeITin mRNA was expressed in mice mammary gland at different lactation stages. Lactoferrin mRNA expressive level decreased as the lactation progressed, its expressive level at early stage of lactation was significantly different from that at middle and later stage of lactation. The results suggested that lactoferrin was involved in the regulation of mammary immunity during the lactation of the tested mice.