一种改良的乳酸菌活菌数快速测定方法

An Improved Method on Determining the Quick Count of Viable Lactobacillus

乳酸菌有多种计数方法,但对科研和生产急需的乳酸菌活菌数快速计数方法的研究和报道很少。文中通过对美蓝直接计数法与平板稀释法、高层琼脂法、GB法、血球计数板计数法、特殊染色计数法、比浊计数法等进行试验比照,特别是美蓝直接计数法与平板稀释法的对比试验,研究出一种直接、快速、准确地测定乳酸菌活菌总数的美蓝直接计数法。

Lactobacillus has several counting methods; however there are few research studies and reports on quick count of viable Lactobacillus. This article compares Methylene Blue direct count method against Plate Dilution method, Plate Count Agar（PCA）method, GB method, Blood Cell counting plate method, Special Dyeing Count method and Turbi- dimetry. Out of all the comparisons, Methylene Blue direct count method against Plate Dilution method confirms that Lacto- bacillus- Methylene Blue direct count method is the fast and accurate way to obtain Lactobacillus total viable count.