黄芪皂甙对豚鼠心室肌细胞动作电位的作用

目的研究黄芪皂甙(saponins of astrag-alus,SA)对豚鼠心室肌细胞动作电位(AP)的作用。方法实验采用标准玻璃微电极细胞内记录心室肌细胞动作电位(AP)。结果应用SA20mg/L后心室肌细胞动作电位的幅度从给药前的(69.31±6.07)mV降低到(57.37±3.68)mV(n=6,P<0.05);动作电位时程(APD90)从给药前的(263.38±16.36)ms延长到(284.33±21.21)ms(n=6,P<0.05)。应用SA40mg/L后心室肌动作电位的幅度从给药前的(71.37±56.34)mV降低到(47.23±8.67)mV(n=6,P<0.05);动作电位时程(APD90)的从给药前的(238.31±13.28)ms延长到(314.64±27.36)ms(n=6,P<0.05)。结论SA可以降低心室肌动作电位幅值延长动作电位时程,并且有一定的剂量依赖性。

Objective To study the effects of saponins of astrag-alus （SA） on the action potential and the delayed rectifier potassium channel in ventricular myocardium of guinea pig Method： The action potential was recorded in guinea pig ventricular myocyte by using of standard microelectrodes technique.Results The action potential amplitude （APA） of ventricular myocardium was decreased from （69.3 1±6.07）mV to （57.37±3.68）mV （n=6,P〈0.05）, the duration （APD90） of ventricular myocardium was expanded from （263.38±16.36）ms to （284.33±21.21）ms （n=6,P〈0.05） after application of SA dosage of 20 mg/L.The APA of ventricular myocardium was decreased from （71.37±56.34）mV to （47.23±8.67） mV （n=6,P〈0.05） and the APD90 of ventricular myocardium was prolonged from （238.31±13.28）ms to （314.64±27.36）ms （n=6,P〈0.05） after application of SA dosage of 40 mg/L. Conclusion It has been suggested that SA decrease action potential amplitude, prolong action potential duration in guinea pig remarkably with concentration-dependence.