系统性红斑狼疮患者外周血中Vα 24^+Vβ11^+、CD161^+Vα 24^+NKT细胞变化及其相关指标的研究

Changes of Vα24^+Vβ11^+,CD161^+Vα24^+NKT Cells in Peripheral Blood of Systemic Lupus Erythematosus Patient

目的研究系统性红斑狼疮患者(SLE)及健康人外周血Vα24+Vβ11+、CD161+Vα24+NKT细胞的数量变化。方法采用流式细胞仪检测30例SLE患者和30例健康对照组Vα24+Vβ11+、CD161+Vα24+NKT细胞数量并与临床指标进行相关分析。结果SLE患者外周血Vα24+Vβ11+、CD161+Vα24+NKT细胞显著低于正常对照组(P<0.05);SLE患者Vα24+Vβ11+、CD161+Vα24+NKT细胞与C3,C4呈正相关关系(P<0.01),与SLE疾病活动性指数(SLEDIA)、红细胞沉降率(ESR)呈负相关关系(P<0.05)。结论CD161+Vα24+NKT显著低于正常对照组,表明SLE患者血中活化的NKT细胞数量减少,SLE患者外周血Vα24+Vβ11+、CD161+Vα24+NKT细胞可能参与SLE的发病过程且与病情活动有关。

Objective To explore the changes of the amount and function of Vα24＋Vβ11＋,CD161＋Vα24＋NKT cells in the peripheral blood of systemic lupus erythematosus（SLE）patients. Methods The amount of Vα24＋Vβ11＋,CD161＋Vα24＋NKT cells in the peripheral blood of 30 SLE cases and 30 healthy persons were detected by flow cytometry. Results Vα24＋Vβ11＋,CD161＋Vα24＋NKT cells in the peripheral blood of SLE patients were significantly lower than that of normal control（P 〈 0.05）. Vα24＋Vβ11＋ and CD161＋Vα24＋NKT cells in the peripheral blood of SLE patients positively correlated with the level of the complement （C3, C4 ） and negatively correlated with SLE disease ac- tivity index （SLEDIA） and ESR. Conclusion Activated NKT cells decreased in the peripheral blood of SLE patients. Vα24＋Vβ11＋ and CD161＋Vα24＋NKT cells might be involved in the pathogenesis and development of SLE.