仙鹤草降糖活性成分研究

Studies on the Lowering Blood Sugar Substances from Agrimony

目的：分离鉴定仙鹤草降糖活性部分的化学成分。方法：采用硅胶柱、Sephadex LH-20柱等常压柱色谱手段进行分离纯化，通过理化常数和光谱分析鉴定其结构。结果：从仙鹤草中分得9个化合物，并鉴定为芹菜素-7-O-β-D-葡萄糖苷（1）、儿茶素（2）、槲皮素（3）、芦丁（4）、山奈酚-3—O-α-L-鼠李糖苷（5）、山奈酚-3—O-β-葡萄糖苷（6）、木犀草素-7—O-β-D-葡萄糖苷（7）、19α，24-二羟基乌苏酸（8）、3，3’-二甲基鞣花酸-4-O-β-D-吡喃葡萄糖苷（9）。结论：化合物1、7—9均为首次从该植物中分离得到。

Objective： To isolate and identify the chemical constituents from the active section lowering blood sugar of agrimony. Methods ： The compounds were separated by repeated silica gel, Sephadex LH-20 and HPLC chromatographies. The structures of compounds isolated were identified by analysis of their spectral data and chemical properties. Results ： Nine compounds were isolated from the active section with lowering blood sugar of agrimony and their structures were identified as apigenin-7-O-β-D-glueopyranoside（ 1 ）, eateehin （ 2 ）, quercetin （ 3 ） , rutin （ 4 ） , kaempferol-3-O-α-L-rhamnoside （ 5 ） , Kampferol-3-O-β-D-glucopyranoside （ 6 ） , luteolin-7-0-β- D-glucopyranosidc （ 7 ）, 19α, 24-dihydroxy ursolic acid （ 8 ）, 3,3 ＇-di-O-rnethyl ellagie acid-4-O-β-D-glueopyranoside （9）. Conclusion ： Compounds 1,7,8,9 are isolated from thia plant for the first time.