穿心莲内酯对实验性糖尿病大鼠血糖的影响及其分子机制

Effects of Angrographolide on Plasma Glucose Level of Experiment Diabetic Rats and Its Molecular Mechanism

目的:研究穿心莲内酯对实验性糖尿病大鼠血糖的影响及其分子机制。方法:大鼠腹腔一次性注射链脲佐菌素(STZ)65 mg/kg制备糖尿病大鼠模型,治疗组用穿心莲内酯灌胃2 w,用分光光度计测定各组血糖、血清超氧化物歧化酶(SOD)活性、丙二醛(MDA)含量;酶免法测定血清胰岛素、快捷免疫组化MaxVisionTM法测定胰腺细胞Bcl-2和Bax表达。结果:与模型组比较,穿心莲内酯组大鼠血糖、血清MDA含量显著降低,血清胰岛素含量、SOD活性显著升高(P<0.01);模型组Bcl-2表达减弱,给药组Bcl-2表达较模型组显著增强(P<0.05)。结论:穿心莲内酯对糖尿病模型大鼠具有抑制胰岛细胞凋亡,降低血糖作用;其机制可能与上调Bcl-2基因表达水平,使SOD活性升高,从而减轻氧自由基,减少脂质过氧化生成有关。

Objective： To investigate the effects of Angrographolide on plasma glucose level of diabetic rats and its molecular mechanism. Methods： The diabetic model rats were established by intraperitoneal injection of streptozotocin （65 mg/kg）. Diabetic rats were treated with Angrographolide for 2 weeks, then the activity of superoxide dismutase （SOD） and the content of malondialdehyde （MDA） in serum were examined and the expressions of Bcl-2 and Box gene of Pancreatic Cells were detected. Results： Compared with model rats, the plasma glucose levels and the serum MDA contents of the Angrographolidetreated rats decreased, serum insulin and activity of SOD increased significatantly （P 〈 0. 01 ）. The expression of Bcl-2 was lower in the model group, while the expression was stronger in the treatment group （ P 〈 0. 05 ）. Conclusion ： Angrographolide can inhibit the apoptosis of islet cells and depress plasma glucose level of diabetic rat model. Its mechanism may be associated with the upregulation of the expression of Bel-2, the increase of the activity of SOD, the decrease of the production of free radicals and lipid eroxidadion.