摘要
目的:研究穿心莲内酯对实验性糖尿病大鼠血糖的影响及其分子机制。方法:大鼠腹腔一次性注射链脲佐菌素(STZ)65 mg/kg制备糖尿病大鼠模型,治疗组用穿心莲内酯灌胃2 w,用分光光度计测定各组血糖、血清超氧化物歧化酶(SOD)活性、丙二醛(MDA)含量;酶免法测定血清胰岛素、快捷免疫组化MaxVisionTM法测定胰腺细胞Bcl-2和Bax表达。结果:与模型组比较,穿心莲内酯组大鼠血糖、血清MDA含量显著降低,血清胰岛素含量、SOD活性显著升高(P<0.01);模型组Bcl-2表达减弱,给药组Bcl-2表达较模型组显著增强(P<0.05)。结论:穿心莲内酯对糖尿病模型大鼠具有抑制胰岛细胞凋亡,降低血糖作用;其机制可能与上调Bcl-2基因表达水平,使SOD活性升高,从而减轻氧自由基,减少脂质过氧化生成有关。
Objective: To investigate the effects of Angrographolide on plasma glucose level of diabetic rats and its molecular mechanism. Methods: The diabetic model rats were established by intraperitoneal injection of streptozotocin (65 mg/kg). Diabetic rats were treated with Angrographolide for 2 weeks, then the activity of superoxide dismutase (SOD) and the content of malondialdehyde (MDA) in serum were examined and the expressions of Bcl-2 and Box gene of Pancreatic Cells were detected. Results: Compared with model rats, the plasma glucose levels and the serum MDA contents of the Angrographolidetreated rats decreased, serum insulin and activity of SOD increased significatantly (P 〈 0. 01 ). The expression of Bcl-2 was lower in the model group, while the expression was stronger in the treatment group ( P 〈 0. 05 ). Conclusion : Angrographolide can inhibit the apoptosis of islet cells and depress plasma glucose level of diabetic rat model. Its mechanism may be associated with the upregulation of the expression of Bel-2, the increase of the activity of SOD, the decrease of the production of free radicals and lipid eroxidadion.
出处
《中药材》
CAS
CSCD
北大核心
2009年第10期1577-1579,共3页
Journal of Chinese Medicinal Materials
基金
广西省教育厅基金资助项目(0626137)
广西科技厅基金项目(0728232)
关键词
穿心莲内酯
糖尿病大鼠
血糖
分子机制
Angrographolide
Diabetic rats
Plasma glucose
Molecular mechanism