苹果胚性细胞的诱导及植株再生研究

Study on the Embryogenic Cells Induction and Plant Regeneration of Apple

分别以苹果胚性组织幼胚子叶和珠心以及非胚性组织离体叶片为试材,对苹果胚性细胞的诱导及植株再生的影响因素进行了研究。结果表明:外植体来源对苹果胚性细胞的诱导及植株再生能力影响显著。虽然胚性组织(幼胚子叶和珠心组织)均可高频率诱导胚性细胞,但其植株再生潜力差。而叶片诱导胚性细胞受培养基中生长调节剂种类和浓度影响明显。在含有2,4-D和BA的MS培养基上,叶片可诱导胚性细胞,胚性细胞在接种于含低浓度BA的分化培养基上可获得较高频率的植株再生。与胚性组织相比,苹果叶片细胞具有较高的全能性,可作为苹果细胞工程中较为理想的细胞来源。

The influencing factors of the embryogenic cells induction and plant regenration of apple were studied by using the embryogenic tissue（immature cotyledons and nucellus）and the non embryogenic tissue（in vito leaf）.The results showed that the embryogenic cells induction and the plant regenration abilities were significantly influenced the eyplant sources.The embryogenic cells could be induced on the embryogenic tissue easily,while the plant regeneration ability of the embryogenic cells was poor.The embryogenic cells induced on leaf were influenced significantly by the type and concentration of growth regulators in the medium.The embryogenic cells could be induced on the in vitro leaf on MS medium with 24-D and BA,and could fruther developed into plantlets on the differentiation medium with low concentration of BA.The cell totipotency of in vitro leaf was higher than that of the embryogenic tissues,which could be used as the ideal cell sources in the apple cell engineering.