多波长RP-HPLC法测定抗感解毒口服液中黄芩苷、绿原酸和栀子苷

Determination of baicalin,chlorogenic acid and jasminoidin in Kangganjiedu Oral Solution by multi-wavelength RP-HPLC

目的建立同时测定抗感解毒口服液中黄芩苷、绿原酸和栀子苷的分析方法。方法采用多波长RP-HPLC法,色谱柱为AgilentZorbaxSBC18柱(250mm×4.6mm,5μm),流动相:0.3%的甲酸水溶液(A)、乙腈(B);线性梯度洗脱,B:14%～20%,0～12min;B:20%～30%,12～13min;B:30%～65%,13～25min;B:65%,25～35min;柱温:30℃;流速:1.0mL/min。结果黄芩苷、绿原酸和栀子苷分别在0.225～3.60μg,0.0230～0.3680μg,0.135～2.16μg的范围内线形关系良好,平均回收率分别为99.62%、99.64%、100.3%,RSD分别为0.68%、1.5%、0.23%。结论本法简便、准确、重现性好,可用于抗感解毒口服液中黄芩苷、绿原酸和栀子苷的同时测定。

Objective To set up a method for determining and analyzing baicalin, chlorogenic acid and jasminoidin in Kangganjiedu Oral Solution simultaneously by multi-wavelength RP-HPLC. Methods The procedure of multi-wavelength RP-HPLC was performed on the chromatographic column of Agilent Zorbax SB C18 （250mm×4.6mm,5μm）. The mobile phase of linear gradient elution was 0.3% aqueous formic acid solution （A） to acetonitrile （B）, and performed as follows： B： 14% -20% ,0 - 12 min; B： 20% -30% ,12-13 min; B： 30% -65% ,13 -25 min; B： 65% ,25-35 min. The flow rate was 1. 0 mL/min. The column temperature was 30 ℃. Results Baicalin showed a good linear relationship at a range of 0. 225 - 3. 60 μg, chlorogenic acid, at 0. 023 0 - 0. 368 0 μg, and jasminoidin, at 0. 135 -2.16 μg. The average recovery of baicalin, chlorogenic acid and jasminoidin was 99.62%, 99.64% and 100.3% respectively. RSD was 0.68%, 1.5% and 0.23% respectively. Conclusion The method was easy and accurate with a higher repeatability, which can be used in the determination of baicalin, chlorogenic acid and jasminoidin in Kangganjiedu Oral Solution simultaneously.