逍遥散对肝郁脾虚证模型大鼠外周促肾上腺皮质激素、皮质酮的调节作用

Effect of Xiaoyaosan on blood ACTH and CORT levels of rat with liver depression and spleen def iciency syndrome

目的:探讨肝郁脾虚证模型大鼠血浆促肾上腺皮质激素(ACTH)、血清皮质酮(CORT)浓度变化及逍遥散对其影响。方法:雄性SD大鼠28只,按体质量随机分为3组:A组,正常对照组8只;B组,模型组10只;C组,逍遥散组10只。B、C组以慢性束缚方法制作肝郁脾虚证模型。自造模第1d开始,C组每日在束缚前1h灌服逍遥散有效组份悬液;A、B组灌服等量蒸馏水。造模21d结束后,各组动物麻醉后取血,ELISA法测血浆ACTH及CORT。结果:给予大鼠慢性束缚21d后,模型组血浆ACTH的值虽较正常对照组升高,但无显著性差异(P>0.05);模型组血清CORT的值较正常对照组明显升高,有显著差异(P<0.05)。逍遥散组ACTH和CORT的值与模型组及正常对照组比较均无显著性差异。结论:肝郁脾虚证模型大鼠血清CORT的含量升高,逍遥散可降低其含量,提示逍遥散疏肝健脾的功效可能对HPA轴具有负反馈调节的作用。

Objective： To observe the changes of blood ACTH and CORT levels of rat with liver depression and spleen deficiency syndrome caused by chronic immobilization stress （CIS）, and discuss protective effect of Xiaoyaosan. Methods： 28 male SD rats were randomly divided into normal control group （ A group ） , model group （ B group ） , Xiaoyansan group （ C group ） . Rats of B,C groups were restrained 3 hours per day for 21days to establish model of liver depression and spleen deficiency syndrome caused by chronic immobilization stress （CIS）. Xiaoyaosan was administered from the first day to the 21st day before the immobilization in C group. Rats were executed on the 22nd day and blood were collected after fast decapitation, contents of plasma ACTH and serum CORT were measured. On day 21, the plasma ACTH level of model group was higher than control group, but there was no obvious difference（P〉0.05）; the serum CORT level of model group was obviously higher than that of control group （P〈0.05）. There were no significant differences in blood ACTH and CORT levels between Xiaoyaosan group and control group, Xiaoyaosan group and model groups. Conclusion： Xiaoyaosan can decrease the levels of blood ACTH and CORT of rat with liver depression and spleen deficiency syndrome, which indicated that Xiaoyaosan had negative feedback regulation on HPA axis.