利胆补肾颗粒对染铅大鼠海马中乙酰胆碱和兴奋性氨基酸的影响

Effect of Lidan Bushen Granules on acetylcholine and excitatory amino acids of hippocampus in saturnine rats

目的:探讨利胆补肾颗粒治疗儿童铅中毒的神经生化机制。方法:经灌胃染铅建立铅中毒动物模型,随机分组后药物干预5周,检测大鼠海马组织中乙酰胆碱(ACH)和兴奋性氨基酸(EAA)的含量。结果:模型组大鼠海马组织中ACH和EAA的含量显著低于正常对照组(P<0.05);利胆补肾颗粒高剂量组、依地酸钙钠组及EDTA和利胆醇联合用药组ACH和EAA含量趋于正常,与模型组比显著升高(P<0.05),其中以利胆补肾颗粒高剂量组疗效最为明显;利胆醇组和利胆补肾颗粒低剂量组则对海马组织中的ACH和EAA含量的影响与模型组比较无显著差异。结论:利胆补肾颗粒改善染铅大鼠学习和记忆能力可能与通过影响海马组织中ACH和EAA的含量有关。

Objective： To discuss the neurobiochemical mechanism of Lidan Bushen Granules in treating saturnine children. Methods： Saturnine rats model was established by intragastric, and was treated with Lidan Bushen Granules for 5 weeks. The acetylcholine and excitatory amino acids of hippocampus in saturnine rats were measured. Results： Compared with the normal group, the ACH and EAA contents of hippocampus in model group rats were dramatically lower （P〈0.05）. Compared with model group, the ACH and EAA contents of hippocampus in rats in Lidan Bushen Granules high-dose group, in EDTA group, in EDTA and Livonal group increased obviously（P〈0.05）. Compared with the model group, the ACH and EAA contents of hippocampus in rats in Livonal group and the Lidan Bushen Granules low-dose group had no significant difference. Conclusion： Lidan Bushen Granules can improve the ability of learning and memory in saturnine rats by affecting the ACH and EAA contents.